Chemical synthesis mono- and diacylglycerol from palm stearin-olein blend using continuous high shear stirred tank reactor

Elma Sulistiya, Rini Yanti, Chusnul Hidayat


This research aimed to evaluate the effect of flow rate and processing time on the synthesis of high mono- and diacylglycerol (MDAG) from palm stearin-olein blend using high shear continuous stirred tank reactor (HS-CSTR). Glycerolysis-interesterification was performed at 120 ºC and flow rates of 6, 10, 14, 18, and 22 mL/min. Glycerol:oil ratio, stearin:olein ratio, NaOH concentration, and agitation rate were 1:5 (mol/mol), 60:40 (w/w), 3%, and 2000 rpm, respectively. The result showed that flow rate significantly affected MDAG concentration and the product's physical characteristics. The highest MDAG was obtained at a flow rate of 6 mL/min. MDAG concentration, slip melting point (SMP), melting point (MP), hardness, emulsion capacity, and stability were 60.36 ± 1.61%, 42.3 ± 0.01 ºC, 43.3 ± 0.06 ºC, 6.04 ± 0.32 N, 87.6 ± 1.75 % and 91.8 ± 2.99 % respectively. An increase in residence time, which means flow rate decreased, increased MDAG, SMP, MP, hardness, emulsion capacity, and stability of the product. Processing time did not significantly affect MDAG concentration and the product's physical properties. It means that acylglycerol concentrations and physical properties of the product did not fluctuate significantly during the process. Thus, it confirmed that the continuous process was stable and reached a steady state throughout the process.


Continuous high shear stirred tank reactor; Diacylglycerol; Monoacylglycerol; Palm stearin-Palm olein blend

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Affandi, A. R., Andarwulan, N., and Haryadi, P. (2017) ‘Pengaruh waktu dan suhu gliserolisis terhadap sifat kimia mono-diasilgliserol pada skala pilot plant (The effect of glycerolysis time and temperature on the chemical properties of mono-diacylglycerol on a pilot plant scale)’, Jurnal Teknologi dan Industri Pangan, 28(2), pp. 159–168 [In Indonesian]

Arum, A. P., Hidayat, C., and Supriyanto. (2019) 'Synthesis of Emulsifier from Refined Bleached Deodorized Palm Stearin by Chemical Glycerolysis in Stirred Tank Reactor’, KnE Life Sciences, 4(11), pp 130-148

Biswas, N., Cheow, Y. L., Tan, C. P., and Siow, L. F. (2017) 'Physical, rheological and sensorial properties, and bloom formation of dark chocolate made with cocoa butter substitute (CBS)', LWT - Food Science and Technology, 82, pp. 420-428

Boukhalkhal, A. L., Kadi, M. E. A., Lasbet, Y., Loubar, K., Awad, S., Makhlouf, M., and Tazerout, M. (2019) ‘A continuous biodiesel production process using a chaotic mixer-reactor’, Waste and Biomass Valorization, 11(11), pp. 6159–6168

Bramasta, D. A., Hidayat, C., Setyaningsih, W. (2019) 'Sintesis Mono- dan Diasilgliserol dari Campuran Stearin Ayam dan Stearin Sawit Menggunakan Centrifugal Contactor Reactor: Kajian Konsentrasi Katalis Sodium Metoksida dan Suhu Gliserolisis (Synthesis of Mono- and Diacylglycerol from a Mixture of Chicken Stearin and Palm Stearin Using a Centrifugal Contactor Reactor: Study of Sodium Methoxide Catalyst Concentration and Glycerolysis Temperature). Thesis. Universitas Gadjah Mada, Yogyakarta. [In Indonesian]

Cole, K. P., and Johnson, M. D. (2018) 'Continuous flow technology vs. the batch-by-batch approach to produce pharmaceutical compounds', Expert Review of Clinical Pharmacology, 11(1), pp. 5–13

Affandi, R. D. N., Aruan, T. R., Taslim., and Iriany (2013) ‘Produksi biodiesel dari lemak sapi dengan proses transesterifikasi dengan katalis basa NaOH (Production of biodiesel from cow fat by transesterification process with NaOH base catalyst)’, Jurnal Teknik Kimia USU, 2(1), pp. 1–6 [In Indonesian]

Florit, F., Busini, V., Storti, G., and Rota, R. (2018) ‘From semi-batch to continuous tubular reactors: A kinetics-free approach’, Chemical Engineering Journal, 354, pp. 1007–1017

De Garmo, E. P., Sullivan, W. G., and Canda, J. R. (1984) Engineering Economy. 7 th. New York: Mc Millan Publ. Co.

Hasibuan, H. A. (2012) ‘Kajian mutu dan karakteristik minyak sawit indonesia serta produk fraksinasinya (Study of the quality and characteristics of indonesian palm oil and its fractionated products)’, Jurnal Standarisasi, 14(2), pp. 98–104 [In Indonesian]

Karouw, S. (2014) ‘Pemanfaatan stearin sawit dan minyak kelapa untuk formulasi asam lemak mirip ASI (Utilization of palm stearin and coconut oil for formulation of fatty acids similar to breast milk)’, Perspektif: Review Penelitian Tanaman Industri, 13(2), pp. 63–74 [In Indonesian]

Lindeque, R. M., and Woodley, J. M. (2019) ‘Reactor selection for effective continuous biocatalytic production of pharmaceuticals’, Catalysts, 9(3), pp. 1-17

Naik, M. K., Naik, S. N., and Mohanty, S. (2014) 'Enzymatic glycerolysis for conversion of sunflower oil to food based emulsifiers', Catalysis Today, 237, pp. 145–149

Putri, S. K., Hariyadi, P., and Andarwulan, N. (2020) ‘Pemurnian produk mono-diasilgliserol ( MDAG ) hasil gliserolisis kimia dengan metode demulsifikasi krim (Product purification of mono-diacylglycerol (MDAG) result of chemical glycerolysis with cream demulsification method)’, AGRITECH, 40(1), pp. 39–47 [In Indonesian]

Rarokar, N. R., Menghani, S., Kerzare, D., and Khedekar, P. J. (2017) ‘Progress in synthesis of monoglycerides for use in food and pharmaceuticals’, Journal of Experimental Food Chemistry, 5(2), pp. 13-19

Rumondang, I., Setyaningsih, D., and Hermanda, A. (2016) ‘Sintesis mono-diasilgliserol berbasis gliserol dan palm fatty acid distillate (Synthesis of Mono-Diacylglycerol Based Glycerol and Palm Fatty Acid Distillate)’, Jurnal Kimia dan Kemasan, 38(1), pp. 1–6

Saberi, A. H., Chin-Ping, T., and Oi-Ming, L. (2011) ‘Phase behavior of palm oil in blends with palm-based diacylglycerol’, Journal of the American Oil Chemists’ Society, 88(12), pp. 1857–1865

Subroto, E., Supriyanto, Utami, T., Hidayat, C. (2018) ‘Enzymatic glycerolysis–interesterification of palm stearin–olein blend for synthesis structured lipid containing high mono- and diacylglycerol’, Food Science and Biotechnology, 28(2), pp. 511–517

Subroto, E. (2020) 'Monoacylglycerols and diacylglycerols for fat-based food products: A review', Food Research, 4(4), pp. 932–943

Zhang, J., Xu, S., and Li, W. (2012) 'High shear mixers: A review of typical applications and studies on power draw, flow pattern, energy dissipation, and transfer properties', Chemical Engineering and Processing: Process Intensification, 57-58, pp. 25–41

Zhang, Z., Ma, X., Huang, H., Li, G., and Wang Y.,(2017) ‘Enzymatic production of highly unsaturated monoacyglycerols and diacylglycerols and their emulsifying effects on the storage stability of a palm oil based shortening system’, Journal of the American Oil Chemists’ Society, 94(9), pp. 1175–1188



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