Bioactive compounds of rice bran and their potential health benefits in the development of functional foods: A review

Miftahurrahmi Miftahurrahmi, Teti Estiasih, Siti Narsito Wulan


Rice bran is a by-product produced from the milling and polishing process of rice, which makes up about 10% of the whole grain. In 2021, the amount of rice bran produced in Indonesia can reach up to 3 million tons. Researchers have been intrigued to study rice bran as an ingredient for foods because it is considered underutilized despite its abundance and functional properties. Rice bran is rich in nutrients such as fat, protein, dietary fiber, ash, and bioactive compounds. There is an increase in the use of rice bran following consumers’ search for healthier consumption. Bioactive compounds in rice bran include phenolic acids, such as ferulic acid; flavonoids and anthocyanins, such as cyanidin glucoside; steroidal compounds, such as gamma-oryzanol; gamma-aminobutyric acid (GABA); etc. Rice bran’s wax contains wax esters and aliphatic alcohols, namely policosanol. Bioactive compounds in rice bran have been known to have beneficial effects on health as antioxidants, antidiabetic, and anti-inflammatory agents. The bioactive compounds of rice bran as well as its antioxidant activity are influenced by rice variety, degree of milling, and processing method. This paper aims to review the bioactive compounds of rice bran, including pigmented rice bran, to support the development of functional foods.


Bioactive; Functional food; Rice bran

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Alauddina, M., Islama, J., Shirakawaa, H., Kosekib, T., Ardiansyahc, and Komaia, M. (2017) ‘Rice bran as a functional food: An overview of the conversion of rice bran into a superfood/functional food’ in Waisundara, V.Y. and Shiomi N. (eds.) Superfood and Functional Food, London: InTech, pp. 291–305

Bhuyan, P., Sarma, S., Ganguly, M., Hazarika, J., and Mahanta, R. (2020) ‘Glutamine : Fructose-6-phosphate aminotransferase (GFAT) inhibitory activity of the anthocyanins present in black rice bran : A probable mechanism for the anti diabetic effect’, Journal of Molecular Structure, 1222, pp 1–8

BPS. (2021) ‘Luas Panen dan Produksi Padi di Indonesia 2021 (Angka Sementara)’. Jakarta: BPS [In Indonesian]

Capellini, M.C., Giacomini, V., Cuevas, M.S., and Rodrigues, C.E.C. (2017) ‘Rice bran oil extraction using alcoholic solvents: Physicochemical characterization of oil and protein fraction functionality’, Industrial Crops and Products, 104, pp. 133–143

Chen, H., Chen, Z., Fu, Y., Liu, J., Lin, S., Zhang, Q., Liu, Y., Wu, D., Lin, D., Han, G., Wang, L., and Qin, W. (2019) ‘Structure, antioxidant, and hypoglycemic activities of arabinoxylans extracted by multiple methods from triticale’, Antioxidants, 8(584), pp. 1–15

Chen, X.Q., Nagao, N., Itani, T., and Irifune, K. (2012) ‘Anti-oxidative analysis, and identification and quantification of anthocyanin pigments in different coloured rice’, Food Chemistry, 135(4), 2783–2788

Chen, Z., Li, S., Fu, Y., Li, C., Chen, D., and Chen, H. (2019) ‘Arabinoxylan structural characteristics, interaction with gut microbiota and potential health functions’, Journal of Functional Foods, 54, pp. 536–551

Chia, S.L., Boo, H.C., Muhamad, K., Sulaiman, R., Umanan, F., and Chong, G.H. (2015) ‘Effect of subcritical carbon dioxide extraction and bran stabilization methods on rice bran oil’, Journal of the American Oil Chemists’ Society, 92(3), pp. 393–402

Das, A.B., Goud, V. V., and Das, C. (2017) ‘Extraction of phenolic compounds and anthocyanin from black and purple rice bran (Oryza sativa L.) using ultrasound: A comparative analysis and phytochemical profiling’, Industrial Crops and Products, 95, pp. 332–341

Deng, G.F., Xu, X.R., Zhang, Y., Li, D., Gan, R.Y., and Li, H. Bin. (2013) ‘Phenolic compounds and bioactivities of pigmented rice’, Critical Reviews in Food Science and Nutrition, 53(3), pp. 296–306

El-Din, N.K.B., Ali, D.A., Othman, R., French, S.W., and Ghoneum, M. (2020) ‘Chemopreventive role of arabinoxylan rice bran, MGN-3/Biobran, on liver carcinogenesis in rats’, Biomedicine and Pharmacotherapy, 126, pp. 1–10

Fabian, C., Ayucitra, A., Ismadji, S., and Ju, Y.H. (2011) ‘Isolation and characterization of starch from defatted rice bran’, Journal of the Taiwan Institute of Chemical Engineers, 42(1), pp. 86–91

Faria, S.A. dos S.C., Bassinello, P.Z., and Penteado, M. de V.C. (2012) ‘Nutritional composition of rice bran submitted to different stabilization procedures’, Brazilian Journal of Pharmaceutical Sciences, 48(4), pp. 652–657

Fraterrigo Garofalo, S., Tommasi, T., and Fino, D. (2021) ‘A short review of green extraction technologies for rice bran oil’, Biomass Conversion and Biorefinery, 11(2), pp. 569–587

Friedman, M. (2013) ‘Rice brans, rice bran oils, and rice hulls: Composition, food and industrial uses, and bioactivities in humans, animals, and cells’, Journal of Agricultural and Food Chemistry, 61(45), pp. 10626–10641

Gasymov, O.K., Celik, S., Agaeva, G., Akyuz, S., Kecel-Gunduz, S., Qocayev, N.M., Ozel, A.E., Agaeva, U., Bakhishova, M., and Aliyev, J.A. (2021) ‘Evaluation of anti-cancer and anti-covid-19 properties of cationic pentapeptide Glu-Gln-Arg-Pro-Arg, from rice bran protein and its D-isomer analogs through molecular docking simulations’, Journal of Molecular Graphics and Modelling, 108, pp. 1–15

Ghasemzadeh, A., Karbalaii, M.T., Jaafar, H.Z.E., and Rahmat, A. (2018) ‘Phytochemical constituents, antioxidant activity, and antiproliferative properties of black, red, and brown rice bran’, Chemistry Central Journal, 12(1), pp. 1–13

Gul, K., Yousuf, B., Singh, A.K., Singh, P., and Wani, A.A. (2015) ‘Rice bran: Nutritional values and its emerging potential for development of functional food - A review’, Bioactive Carbohydrates and Dietary Fibre, 6(1), 24–30

Halee, A., Supavititpatana, P., Ruttarattanamongkol, K., Jittrepotch, N., Rojsuntornkitti, K., and Kongbangkerd, T. (2020) ‘Optimisation of the microwave-assisted extraction of natural antioxidants from defatted black rice bran of Oryza sativa L. CV. homnin’, Journal of Microbiology, Biotechnology and Food Sciences, 9(6), pp. 1134–1140

Heidtmann-Bemvenuti, R., Nora, N.S., and Badiale-Furlong, E. (2012) ‘Extraction of γ -oryzanol from rice bran’, Ciencia e Agrotecnologia, 36(6), pp. 665–673

Hu, E.A., Pan, A., Malik, V., and Sun, Q. (2012) ‘White rice consumption and risk of type 2 diabetes: Meta-analysis and systematic review’, British Medical Journal, 344, pp. 1–9

Hu, Z., Qiu, L., Sun, Y., Xiong, H., and Ogra, Y. (2019) ‘Improvement of the solubility and emulsifying properties of rice bran protein by phosphorylation with sodium trimetaphosphate’, Food Hydrocolloids, 96, pp. 288–299

Huang, Y.P., and Lai, H.M. (2016) ‘Bioactive compounds and antioxidative activity of colored rice bran’, Journal of Food and Drug Analysis, 24(3), pp. 564–574

Ishaka, A., Imam, M.U., Mahamud, R., Zuki, A.B.Z., and Maznah, I. (2014) ‘Characterization of rice bran wax policosanol and its nanoemulsion formulation’, International Journal of Nanomedicine, 9, pp. 2261–2269

Jayachandran, M., Chen, J., Chung, S.S.M., and Xu, B. (2018) ‘A critical review on the impacts of β-glucans on gut microbiota and human health’, Journal of Nutritional Biochemistry, 61, pp. 101–110

Jongsomchai, K., Leardkamolkarn, V., and Mahatheeranont, S. (2020) ‘A rice bran phytochemical, cyanidin 3-glucoside, inhibits the progression of PC3 prostate cancer cell’, Anatomy & Cell Biology, 53(4), pp. 481–492

Jung, T.D., Shin, G.H., Kim, J.M., Choi, S. Il, Lee, J.H., Lee, S.J., Park, S.J., Woo, K.S., Oh, S.K., and Lee, O.H. (2017) ‘Comparative analysis of γ-oryzanol, β-glucan, total phenolic content and antioxidant activity in fermented rice bran of different varieties’, Nutrients, 9(6), pp. 1–12

Justo, M.L., Rodriguez–Rodriguez, R., Claro, C.M., Alvarez De Sotomayor, M., Parrado, J., and Herrera, M.D. (2013) ‘Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese Zucker rats’, European Journal of Nutrition, 52(2), pp. 789–797

Kalpanadevi, C., Singh, V., and Subramanian, R. (2018) ‘Influence of milling on the nutritional composition of bran from different rice varieties’, Journal of Food Science and Technology, 55(6), pp. 2259–2269

Kaur, A., Virdi, A.S., Singh, N., Singh, A., and Kaler, R.S.S. (2021) ‘Effect of degree of milling and defatting on proximate composition, functional and texture characteristics of gluten-free muffin of bran of long-grain indica rice cultivars’, Food Chemistry, 345, pp. 1–13

Kim, H.S., Lee, E.J., Lim, S.T., and Han, J.A. (2015) ‘Self-enhancement of GABA in rice bran using various stress treatments’, Food Chemistry, 172, pp. 657–662

Kim, J.Y., Seo, W.D., Park, D.S., Jang, K.C., Choi, K.J., Kim, S.Y., Oh, S.H., Ra, J.E., Yi, G., Park, S.K., Hwang, U.H., Song, Y.C., Park, B.R., Park, M.J., Kang, H.W., Nam, M.H., and Han, S.I. (2013) ‘Comparative studies on major nutritional components of black waxy rice with giant embryos and its rice bran’, Food Science and Biotechnology, 22, pp. 121–128

Kim, S.M., Chung, H.J., and Lim, S.T. (2014) ‘Effect of various heat treatments on rancidity and some bioactive compounds of rice bran’, Journal of Cereal Science, 60(1), pp. 243–248

Lemus, C., Angelis, A., Halabalaki, M., and Skaltsounis, A.L. (2014) ‘Chapter 32 - γ-Oryzanol: An Attractive Bioactive Component from Rice Bran’ in Watson, C., Preedy, V.R., and Zibadi S. (eds.) Wheat and Rice in Disease Prevention and Health, Elsevier Inc., pp. 409-430

Li, S., Chen, H., Cheng, W., Yang, K., Cai, L., He, L., Du, L., Liu, Y., Liu, A., Zeng, Z., and Li, C. (2021) ‘Impact of arabinoxylan on characteristics, stability and lipid oxidation of oil-in-water emulsions: Arabinoxylan from wheat bran, corn bran, rice bran, and rye bran’, Food Chemistry, 358, pp. 1–12

Liang, Y., Gao, Y., Lin, Q., Luo, F., Wu, W., Lu, Q., and Liu, Y. (2014) ‘A review of the research progress on the bioactive ingredients and physiological activities of rice bran oil’, European Food Research and Technology, 238(2), pp. 169–176

Liu, R., Xu, Y., Chang, M., Tang, L., Lu, M., Liu, Ruijie, Jin, Q., and Wang, X. (2021) ‘Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil’, Food Chemistry, 343, pp. 1–8

Liu, Y., Zhang, H., Yi, C., Quan, K., and Lin, B. (2021) ‘Chemical composition, structure, physicochemical and functional properties of rice bran dietary fiber modified by cellulase treatment’, Food Chemistry, 342, pp. 1–9

Liu, Y.Q., Strappe, P., Zhou, Z.K., and Blanchard, C. (2019) ‘Impact on the nutritional attributes of rice bran following various stabilization procedures’, Critical Reviews in Food Science and Nutrition, 59(15), pp. 2458–2466

Luo, Y., Li, Z., Li, X., Liu, X., Fan, J., Clark, J.H., and Hu, C. (2019) ‘The production of furfural directly from hemicellulose in lignocellulosic biomass: A review’, Catalysis Today, 319, pp. 14–24

Mineo, S., Takahashi, N., Yamada-Hara, M., Tsuzuno, T., Aoki-Nonaka, Y., and Tabeta, K. (2021) ‘Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells’, Archives of Oral Biology, 129, pp. 1–8

Moongngarm, A., Daomukda, N., and Khumpika, S. (2012) ‘Chemical compositions, phytochemicals, and antioxidant capacity of rice bran, rice bran layer, and rice germ’, APCBEE Procedia, 2, pp. 73–79

Nandi, I., Sengupta, A., and Ghosh, M. (2019) ‘Effects of dietary fibers extracted from defatted sesame husk, rice bran & flaxseed on hypercholesteremic rats’, Bioactive Carbohydrates and Dietary Fibre, 17, pp. 1–9

Nisa, K., Rosyida, V.T., Nurhayati, S., Indrianingsih, A.W., Darsih, C., and Apriyana, W. (2019) ‘Total phenolic contents and antioxidant activity of rice bran fermented with lactic acid bacteria’, IOP Conference Series: Earth and Environmental Science, 251(1), pp. 1–9

Ooi, S.L., Pak, S.C., Micalos, P.S., Schupfer, E., Zielinski, R., Jeffries, T., Harris, G., Golombick, T., and McKinnon, D. (2020) ‘Rice bran arabinoxylan compound and quality of life of cancer patients (RBAC-QoL): Study protocol for a randomized pilot feasibility trial’, Contemporary Clinical Trials Communications, 19, pp. 1–9

Paiva, F.F., Vanier, N.L., Berrios, J.D.J., Pan, J., Villanova, F. de A., Takeoka, G., and Elias, M.C. (2014) ‘Physicochemical and nutritional properties of pigmented rice subjected to different degrees of milling’, Journal of Food Composition and Analysis, 35(1), pp. 10–17

Peanparkdee, M., Patrawart, J., and Iwamoto, S. (2019) ‘Effect of extraction conditions on phenolic content, anthocyanin content and antioxidant activity of bran extracts from Thai rice cultivars’, Journal of Cereal Science, 86, pp. 86–91

Pengkumsri, N., Chaiyasut, C., Saenjum, C., Sirilun, S., Peerajan, S., Suwannalert, P., Sirisattha, S., and Sivamaruthi, B.S. (2015) ‘Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand’, Food Science and Technology, 35(2), pp. 331–338

Pengkumsri, N., Chaiyasut, C., Sivamaruthi, B.S., Saenjum, C., Sirilun, S., Peerajan, S., Suwannalert, P., Sirisattha, S., Chaiyasut, K., and Kesika, P. (2015) ‘The influence of extraction methods on composition and antioxidant properties of rice bran oil’, Food Science and Technology, 35(3), pp. 493–501

Pérez-Martínez, A., Valentín, J., Fernández, L., Hernández-Jiménez, E., López-Collazo, E., Zerbes, P., Schwörer, E., Nuñéz, F., Martín, I.G., Sallis, H., Díaz, M.Á., Handgretinger, R., and Pfeiffer, M.M. (2015) ‘Arabinoxylan rice bran (MGN-3/Biobran) enhances natural killer cell-mediated cytotoxicity against neuroblastoma invitro and invivo’, Cytotherapy, 17(5), pp. 601–612

Perez-Ternero, C., Werner, C.M., Nickel, A.G., Herrera, M.D., Motilva, M.J., Böhm, M., Alvarez de Sotomayor, M., and Laufs, U. (2017) ‘Ferulic acid, a bioactive component of rice bran, improves oxidative stress and mitochondrial biogenesis and dynamics in mice and in human mononuclear cells’, Journal of Nutritional Biochemistry, 48, pp. 51–61

Phan, V.M., Tran, H.C., and Sombatpraiwan, S. (2021) ‘Rice bran oil extraction with mixtures of ethanol and hexane’, Songklanakarin Journal of Science and Technology, 43(3), pp. 630–637

Phongthai, S., Homthawornchoo, W., and Rawdkuen, S. (2017) ‘Preparation, properties and application of rice bran protein: A review’, International Food Research Journal, 24(1), pp. 25–34

Pokkanta, P., Sookwong, P., Tanang, M., Setchaiyan, S., Boontakham, P., and Mahatheeranont, S. (2019) ‘Simultaneous determination of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone in rice bran and vegetable oil samples’, Food Chemistry, 271, pp. 630–638

Ramos, A.H., Timm, N. da S., Rockenbach, B.A., Ferreira, C.D., Hoffmann, J.F., and Oliveira, M. de. (2022) ‘Red rice drying and storage: Effects on technological properties and phenolic compounds of the raw and cooked grains’, Journal of Cereal Science, 103, pp. 1–9

Saleh, A.S.M., Wang, P., Wang, N., Yang, L., and Xiao, Z. (2019) ‘Brown rice versus white rice: nutritional quality, potential health benefits, development of food products, and preservation technologies’, Comprehensive Reviews in Food Science and Food Safety, 00, pp. 1–27

Setyaningsih, W., Saputro, I.E., Palma, M., and Barroso, C.G. (2015) ‘Optimisation and validation of the microwave-assisted extraction of phenolic compounds from rice grains’, Food Chemistry, 169, pp. 141–149

Sharif, M.K., Butt, M.S., Anjum, F.M., and Khan, S.H. (2014) ‘Rice bran: A novel functional ingredient’, Critical Reviews in Food Science and Nutrition, 54(6), pp. 807–816

Shen, J., Luo, F., and Lin, Q. (2019) ‘Policosanol : Extraction and biological functions’, Journal of Functional Foods, 57, pp. 351–360

Shibata, A., Kawakami, Y., Kimura, T., Miyazawa, T., and Nakagawa, K. (2016) ‘α-Tocopherol attenuates the triglyceride- and cholesterol-lowering effects of rice bran tocotrienol in rats fed a western diet’, Journal of Agricultural and Food Chemistry, 64(26), pp. 5361–5366

Shobako, N., and Ohinata, K. (2020) ‘Anti-hypertensive effects of peptides derived from rice bran protein’, Nutrients, 12(10), pp. 1–11

Wang, N., Chen, J., Zhou, Q., Jiang, L., Wang, L., Dai, Y., Yu, D., and Elfalleh, W. (2021) ‘Crude wax extracted from rice bran oil improves oleogel properties and oxidative stability’, European Journal of Lipid Science and Technology, 123(6), pp. 1–8

Wang, W., Guo, J., Zhang, J., Peng, J., Liu, T., and Xin, Z. (2015) ‘Isolation, identification and antioxidant activity of bound phenolic compounds present in rice bran’, Food Chemistry, 171, pp. 40–49

Wang, X., Chen, H., Fu, X., Li, S., and Wei, J. (2017) ‘A novel antioxidant and ACE inhibitory peptide from rice bran protein: Biochemical characterization and molecular docking study’, LWT - Food Science and Technology, 75, pp. 93–99

Wattanasiritham, L., Theerakulkait, C., Wickramasekara, S., Maier, C.S., and Stevens, J.F. (2016) ‘Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein’, Food Chemistry, 192, pp. 156–162

Xu, D., Hao, J., Wang, Z., Liang, D., Wang, J., Ma, Y., and Zhang, M. (2021) ‘Physicochemical properties, fatty acid compositions, bioactive compounds, antioxidant activity and thermal behavior of rice bran oil obtained with aqueous enzymatic extraction’, Lwt, 149, pp. 1–8

Yao, W., Gong, Y., Li, L., Hu, X., and You, L. (2022) ‘The effects of dietary fibers from rice bran and wheat bran on gut microbiota: An overview’, Food Chemistry: X, 13, pp. 1–11

Zhang, R., Ma, Q., Tong, X., Liu, L., Dong, L., Huang, F., Deng, Y., Jia, X., Chi, J., and Zhang, M. (2020) ‘Rice bran phenolic extract supplementation ameliorates impaired lipid metabolism in high-fat-diet fed mice through AMPK activation in liver’, Journal of Functional Foods, 73, pp. 1–9

Zhang, S., Ma, Q., Dong, L., Jia, X., Liu, L., Huang, F., Liu, G., Sun, Z., Chi, J., Zhang, M., and Zhang, R. (2022) ‘Phenolic profiles and bioactivities of different milling fractions of rice bran from black rice’, Food Chemistry, 378, pp. 1–9

Zhang, X., Shen, Y., Prinyawiwatkul, W., King, J.M., and Xu, Z. (2013) ‘Comparison of the activities of hydrophilic anthocyanins and lipophilic tocols in black rice bran against lipid oxidation’, Food Chemistry, 141(1), pp. 111–116

Zhao, G., Hu, M., Lu, X., and Zhang, R. (2022) ‘Soaking, heating and high hydrostatic pressure treatment degrade the flavonoids in rice bran’, Lwt, 154, pp. 1–9

Zhao, G., Zhang, R., Dong, L., Huang, F., Liu, L., Deng, Y., Ma, Y., Zhang, Y., Wei, Z., Xiao, J., and Zhang, M. (2018) ‘A comparison of the chemical composition, in vitro bioaccessibility and antioxidant activity of phenolic compounds from rice bran and its dietary fibres’, Molecules, 23(1), pp. 1–14


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