The potential of the addition of Secang wood extract on physico-chemical properties, antioxidant and antibacterial activities of goat-milk kefir as a functional food product

Rizka Mulyani, Nurliyani Nurliyani, Indratiningsih Indratiningsih, Prakoso Adi

Abstract


This study aimed to improve the functional quality of goat-milk kefir by adding secang wood extract. The initial stage of the research was the extraction of secang wood using distilled water. Kefir is made by mixing secang wood extract and pasteurized goat milk with 5% kefir grains, then incubated at 25̊C for 20 hours. Goat-milk kefir was then tested for physicochemical quality (i.e., total solids, water content, pH value, acidity value, viscosity, and alcohol content), microbiological quality (i.e., total lactic acid bacteria (LAB) and total yeast), organoleptic properties, antimicrobial activity against Escherichia coli and Staphylococcus aureus, and antioxidant activity. The results showed that the physicochemical quality of kefir with the addition of secang wood extract had an effect (p<0.05) on total solids, water content, pH value, acidity value, and viscosity. The results showed that the addition of secang wood extract 8% to goat-milk kefir has an antioxidant activity of 12.18% and an antibacterial activity by inhibiting Escherichia coli and Staphylococcus aureus 24.81mm and 18.95mm, respectively. These findings confirmed that goat-milk kefir, with the addition of secang wood extract, has the potential as a functional food.


Keywords


Antimicrobial activity; Antioxidant activity; Kefir; Goat milk; Sappan wood

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References


Adi, P., Mulyani, R., and Yang, J. J. (2022) ‘Skrining novel prebiotik selective fermentation initiator (SFI) untuk bakteri probiotik elektrogenik kulit’, Journal of Applied Agriculture, Health and Technology, 1(1), pp. 1-17

Adirestuti, P., Puspadewi, R., Mardatillah, A., Anggita, D., Helena, M., Octavia, A., and Putri, Y. (2018) ‘Optimization of extraction from sappan wood and its influence on food bacterial contaminants’, Indonesian Journal of Pharmaceutical Science and Technology Journal Homepage, 1(1), pp. 21–24

Andrei, S., Bunea, A., Pintea, A., Matei, S., and Cozma, A. M. (2015) ‘Comparative study of antioxidant activity in fresh and pasteurized goat milk’, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 72(2), pp. 336–340

AOAC (1995) Official Methods of Analysis. Washington DC: Association of Official Analytical Chemis.

Badem, A., and Uçar, G. (2017) ‘Production of caseins and their usages’, International Journal of Food Science and Nutrition, 2(1), pp. 2455–4898

Bengoa, A. A., Iraporda, C., Garrote, G. L., and Abraham, A. G. (2019) ‘Kefir micro-organisms: Their role in grain assembly and health properties of fermented milk’, Journal of Applied Microbiology, 126(3), pp. 686–700

Bintsis, T. (2018) ‘Lactic acid bacteria as starter cultures: An update in their metabolism and genetics’, AIMS Microbiology, 4(4), pp. 665–684

Chen, C., Zhao, S., Hao, G., Yu, H., Tian, H., and Zhao, G. (2017) ‘Role of lactic acid bacteria on the yogurt flavour: A review’, International Journal of Food Properties, 20(S1), pp. 316–330

Chu, M. J., Wang, Y. Z., Itagaki, K., Ma, H. X., Xin, P., Zhou, X. G., Chen, G. Y., Li, S., and Sun, S. Q. (2013) ‘Identification of active compounds from Caesalpinia sappan L. extracts suppressing IL-6 production in RAW 264.7 cells by PLS’, Journal of Ethnopharmacology, 148(1), pp. 37–44

Djaeni, M., Kumoro, A. C., Utari, F. D., and Septiani, I. E. (2021) ‘Enhancement of the sappanwood extract yield by aqueous ultrasound-assisted extraction using water solvent’, International Journal on Advanced Science, Engineering and Information Technology, 11(4), pp. 1514–1520

Endah, S. R. N., Wulasari, P. D., Nofriyaldi, A., Nurliyani, and Harmayani, E. (2022) ‘The composition of goat milk kefir added with Moringa oleifera leaf powder’, IOP Conference Series: Earth and Environmental Science, pp. 1-5.

Fadhilah, T. M., Utami, A. N., Puspitadewi, A., Ermawan, A. M., and Dariani, A. P. (2022) ‘The addition of beetroot (Beta vulgaris L.) to dawet ayu sappan wood (Caesalpinia sappan L.) as a functional drink containing anthocyanins’, Sainstek : Jurnal Sains dan Teknologi, 14(2), pp. 2580–278

Farag, M. A., Jomaa, S. A., El-wahed, A. A., and El-seedi, H. R. (2020) ‘The many faces of kefir fermented dairy products: Quality characteristics, flavour chemistry, nutritional value, health benefits, and safety’, Nutrients, 12(2), pp. 1-23

Flieger, J., Flieger, W., Baj, J., and Maciejewski, R. (2021) ‘Antioxidants: Classification, natural sources, activity/capacity measurements, and usefulness for the synthesis of nanoparticles’, Materials, 14(15), pp. 1–54

Galdiero, S., Falanga, A., Cantisani, M., Tarallo, R., Pepa, M. E. D., D’oriano, V., and Galdiero, M. (2012) ‘Microbe-host interactions: Structure and role of gram-negative bacterial porins’, Current Protein and Peptide Science, 13(8), pp. 843–854

Hendarto, D. R., Handayani, A. P., Esterelita, E., and Handoko, Y. A. (2019) ‘Mekanisme biokimiawi dan optimalisasi Lactobacillus bulgaricus dan Streptococcus thermophilus dalam pengolahan yoghurt yang berkualitas (Biochemistry mechanism and optimization Lactobacillus bulgaricus and Streptococcus thermophilus in processing quality yoghurt)’, Jurnal Sains Dasar, 8(1), pp. 13-19 [In Indonesian]

Huang, Z., Huang, L., Xing, G., Xu, X., Tu, C. and Dong, M. (2020) ‘Effect of co-fermentation with lactic acid bacteria and K. Marxianus on physicochemical and sensory properties of goat milk’, Foods, 9(3), pp. 1–14

Husnaeni, Maruddin, F., Malaka, R., and Prahesti, K. I. (2019) ‘Study on the use of various concentration of acetic acid and different precipitation duration on casein characteristics’, IOP Conference Series: Earth and Environmental Science, pp. 1–5.

Irawan, E. W., Sipahelut, S. G., and Mailoa, M. (2022) ‘Potensi ekstrak kayu secang (Caesalpinia sappan L.) sebagai pewarna alami dalam pembuatan selai pala (Myristica fragrans H.) (Potential of sappan wood extract (Caesalpinia sappan L.) as a natural coloring in making nutmeg jam (Myristica fragrans H.))’, Jurnal Teknologi Hasil Pertanian, 15(1), pp. 74–82 [In Indonesian]

Karkadova, M., and Ghoddusi, H. (2018) ‘Fermented milk kefir: probiotic content and potential health benefits’, 7th Annual congress on Probiotics, Nutrition and Microbes, pp. 29

Kesenkaş, H., Yerlikaya, O., and Özer, E. (2015) ‘A functional milk beverage: Kefir’, Agro FOOD Industry Hi Tech, 24(6), pp. 53–55

Kimestri, A. B., Indratiningsih, and Widodo (2018) ‘Microbiological and physicochemical quality of pasteurized milk supplemented with sappan wood extract (Caesalpinia sappan L.)’, International Food Research Journal , 25(1), pp. 392–398

Kimestri, A. B., Indratiningsih, and Widodo (2018) ‘Microbiological and physicochemical quality of pasteurized milk supplemented with sappan wood extract (Caesalpinia sappan L.)’, International Food Research Journal, 25(1), pp. 392–398

Kondo, S., Temrangsee, P., Sattaponpan, C., and Itharat, A. (2017) ‘Antibacterial activity of thai herbal plants and development of hand washing gel product’, Journal of the Medical Association of Thailand, 100(5), pp. 18–22

Leite, A. M. O., Leite, D. C. A., Del Aguila, E. M., Alvares, T. S., Peixoto, R. S., Miguel, M. A. L., Silva, J. T., and Paschoalin, V. M. F. (2013) ‘Microbiological and chemical characteristics of Brazilian kefir during fermentation and storage processes’, Journal of Dairy Science, 96(7), pp. 4149–4159

Listiawati, M., Kurniati, T., and Windayani, N. (2019) ‘An effective alternative of pre-dawn meal using milk kefir’, Journal of Physics: Conference Series, pp. 1–5.

Liu, J. R., Chen, M. J., and Lin, C. W. (2005) ‘Antimutagenic and antioxidant properties of milk-kefir and soymilk-kefir’, Journal of Agricultural and Food Chemistry, 53(7), pp. 2467–2474

Luang-In, V., and Deeseenthum, S. (2016) ‘Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities’, LWT - Food Science and Technology, 73, pp. 592–601

Martharini, D., and Indratiningsih, I. (2017) ‘Kualitas mikrobiologis dan kimiawi kefir susu kambing dengan penambahan Lactobacillus acidophilus FNCC 0051 dan tepung kulit pisang kepok (Musa Paradisiaca) (Microbiological and chemical quality of goat's milk kefir with the addition of Lactobacillus acidophilus FNCC 0051 and kepok banana peel flour (Musa Paradisiaca))’, Agritech, 37(1), pp. 23–29 [In Indonesian]

Mazaya, G., Karseno, K., and Yanto, T. (2020) ‘Antimicrobial and phytochemical activity of coconut shell extracts’, Turkish Journal of Agriculture - Food Science and Technology, 8(5), pp. 1090–1097

Moreno, M. C. R., and Emata, O. C. (2020) ‘Chemical and microbiological properties of kefir produced by kefir grains in raw and pasteurized cow’s milk’, Mindanao Journal of Science and Technology, 18(2), pp. 277–292

Muelas, R., Romero, G., Díaz, J. R., Monllor, P., Fernández-López, J., Viuda-Martos, M., Cano-Lamadrid, M., and Sendra, E. (2022) ‘Quality and functional parameters of fermented milk obtained from goat milk fed with broccoli and artichoke plant by-products’, Foods, 11(17), pp. 1-29

Nayik, G. A., Jagdale, Y. D., Gaikwad, S. A., Devkatte, A. N., Dar, A. H., and Ansari, M. J. (2022) ‘Nutritional profile, processing and potential products: A comparative review of goat milk’, Dairy, 3(3), pp. 622–647

Ngamwonglumlert, L., Devahastin, S., Chiewchan, N., and Raghavan, G. S. V. (2020) ‘Color and molecular structure alterations of brazilein extracted from Caesalpinia sappan L. under different pH and heating conditions’, Scientific Reports, 10(1), pp. 1-10

Nirmal, N. P., and Panichayupakaranant, P. (2015) ‘Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract’, Pharmaceutical Biology, 53(9), pp. 1339–1343

Nurliyani, Sadewa, A. H., and Sunarti (2015) ‘Kefir properties prepared with goat milk and black rice (Oryza sativa L.) extract and its influence on the improvement of pancreatic β-cells in diabetic rats’, Emirates Journal of Food and Agriculture, 27(10), pp. 727–735

Nurliyani, Harmayani, E., and Sunarti (2014) ‘Microbiological quality, fatty acid and amino acid profiles of kefir produced from combination of goat and soy milk’, Pakistan Journal of Nutrition, 13(2), pp. 107–115

Öner, Z., Karahan, A. G., and Çakmakçı, M. L. (2010) ‘Effects of different milk types and starter cultures on kefir’, GIDA, 35(3), pp. 177–182

Palasuwan, A., Soogarun, S., Lertlum, T., Pradniwat, P., and Wiwanitkit, V. (2005) ‘Inhibition of heinz body induction in an in vitro model and total antioxidant activity of medicinal Thai plants’, Asian Pacific Journal of Cancer Prevention, 6(4), pp. 458–463

Payet, B., Sing, A. S. C., and Smadja, J. (2005) ‘Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: Determination of their polyphenolic and volatile constituents’, Journal of Agricultural and Food Chemistry, 53(26), pp. 10074–10079

Periadnadi, P., Sari, D. K., and Nurmiati, N. (2018) ‘Isolasi dan keberadaan khamir potensial pemfermentasi nira aren (Arenga Pinnata Merr.) dari dataran rendah dan dataran tinggi di Sumatera Barat (Isolation and presence of potential yeast fermenting palm sap (Arenga Pinnata Merr.) from the lowlands and highlands in West Sumatra)’, Bioeksperimen, 4(1), pp. 29–36 [In Indonesian]

Ponomarova, O., Gabrielli, N., Sévin, D. C., Mülleder, M., Zirngibl, K., Bulyha, K., Andrejev, S., Kafkia, E., Typas, A., Sauer, U., Ralser, M., and Patil, K. R. (2017) ‘Yeast creates a niche for symbiotic lactic acid bacteria through nitrogen overflow’, Cell Systems, 5(4), pp. 345-357

Raharjo, A. R., Neswati, S., and Siswardjono (2016) Pengaruh Penambahan Maltodekstrin Terhadap Karakteristik Minuman Serbuk Instan Kayu Secang (Caesalpinia sappan, L.) (Effect of Adding Maltodextrin on the Characteristics of Secang Wood Instant Powder Drink (Caesalpinia sappan, L.)). Undergraduate Thesis. Universitas Andalas, Padang. [In Indonesian]

Rahmawati, I. S., and Suntornsuk, W. (2016) ‘Effects of fermentation and storage on bioactive activities in milks and yoghurts’, Procedia Chemistry, 18, pp. 53–62

Rina, O., Sanusilbrahim, AbdiDharma, Afrizal, Utami, C. W., and Widodo, Y. R. (2017) ‘Stabilities natural colorant of Sappan wood (Caesalpinia sappan.. L) for food and beverages in various pH, temperature, and matrices of food’, International Journal of ChemTech Research, 10(1), pp. 98–103

Safitri, R., Siahaan, M., and Ghozali, M. (2022) ‘Antimicrobial activity of Sappan wood extract (Caesalpinia sappan L.) against Streptococcus pneumoniae’, International journal of health sciences, pp. 7881–7896

Setyawardani, T., Rahardjo, A. H., Sulistyowati, M., and Wasito, S. (2014) ‘Physiochemical and organoleptic features of goat milk kefir made of different kefir grain concentration on controlled fermentation’, Animal Production, 16(1), pp. 48–54

Setyawardani, T., Sumarmono, J., Rahardjo, A. H. D., Widayaka, K., and Santosa, R. S. S. (2019) ‘Texture profile and sensory characteristics of kefir with colostrum addition’, IOP Conference Series: Earth and Environmental Science, pp. 1–6

Sinsawasdi, V. K. (2012) Sappanwood Water Extract: Evaluation of Color Properties, Functional Properties, and Toxicity. Dissertation University of Florida, Florida.

Srinivasan, R., Selvam, G. G., Karthik, S., Mathivanan, K., Baskaran, R., Karthikeyan, M., Gopi, M., and Govindasamy, C. (2012) ‘In vitro antimicrobial activity of Caesalpinia sappan L.’, Asian Pacific Journal of Tropical Biomedicine, 2(1), pp. 136-139

Stadie, J., Gulitz, A., Ehrmann, M. A., and Vogel, R. F. (2013) ‘Metabolic activity and symbiotic interactions of lactic acid bacteria and yeasts isolated from water kefir’, Food Microbiology, 35(2), pp. 92–98

Suhartanti, D. (2014) ‘Perbandingan aktivitas antibakteri kefir susu sapi dan kefir susu kambing terhadap bakteri Staphylococcus aureus (Comparison of the antibacterial activity of cow's milk kefir and goat's milk kefir against Staphylococcus aureus bacteria)’, Ekosains, 6(1), pp. 1-7 [In Indonesian]

Sulmiyati, Said, N., Fahrodi, D. U., Malaka, R., and Maruddin, F. (2019) ‘Assessment of the antibacterial activity of goat milk kefir on Escherichia coli ATCC 8739 and Salmonella enteric subsp. enterica serovar typhimurium ATCC 14028 using a well diffusion method’, IOP Conference Series: Earth and Environmental Science, pp. 1-8

Sulmiyati, Said, N. S., Fahrodi, D. U., Malaka, R., and Maruddin, F. (2019) ‘The physicochemical, microbiology, and sensory characteristics of Kefir Goat Milk with different levels of Kefir Grain’, Tropical Animal Science Journal, 42(2), pp. 152–158

Suriasih, K., Aryanta, W. R., Mahardika, G., and Astawa, N. M. (2012) ‘Microbiological and chemical properties of kefir made of bali cattle milk’, Food Science and Quality Management, 6, pp. 12–23

Suwan, T., Wanachantararak, P., Khongkhunthian, S., and Okonogi, S. (2018) ‘Antioxidant activity and potential of Caesalpinia sappan aqueous extract on synthesis of silver nanoparticles’, Drug discoveries & therapeutics, 12(5), pp. 259–266

Turkmen, N. (2017) ‘The nutritional value and health benefits of goat milk components’ in Watson, R. R., Collier, R. J., Preedy, V. R. (eds.) Nutrients in Dairy and their Implications for Health and Disease. Amsterdam: Elsevier, pp. 441–449.

Utari, F. D., Sumirat and Djaeni, M. (2017) ‘Produksi antioksidan dari ekstrak kayu secang (Caesalpinia sappan L.) menggunakan pengering berkelembaban rendah (Production of antioxidants from secang wood extract (Caesalpinia sappan L.) using a low humidity dryer)’, Jurnal Aplikasi Teknologi Pangan, 6(3), pp. 1–4 [In Indonesian]

Vardhani, A.K. (2019) ‘Caesalpinia sappan L: Review article’, Proceedings of International Conference on Applied Science and Health, pp. 300–305

Widowati, W. (2011) ‘Uji fitokimia dan potensi antioksidan ekstrak etanol kayu secang (Caesalpinia sappan L.) (Phytochemical test and antioxidant potential of ethanol extract of secang wood (Caesalpinia sappan L.))’, Maranatha Journal of Medicine and Health, 11(1), pp. 23–31 [In Indonesian]

Xu, H. X., and Lee, S. F. (2004) ‘The antibacterial principle of Caesalpina sappan’, Phytotherapy Research, 18(8), pp. 647–651

Yadav, A., Kumari R., Mishra, J. P., Srivastava, S., and Prabha, S. (2016) ‘Antioxidants and its functions in human body - A Review’, Research in Environment and Life Sciences, 9(11), pp. 1328–1331

Yilmaz-Ersan, L., Ozcan, T., Akpinar-Bayizit, A., and Sahin, S. (2016) ‘The antioxidative capacity of kefir produced from goat milk’, International Journal of Chemical Engineering and Applications, 7(1), pp. 22–26




DOI: https://doi.org/10.21776/ub.afssaae.2023.006.03.7

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